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The increase in duration for the disease-associated HLA-B27 molecules to assemble appears to subsequently lead to the accumulation of misfolded HLA-B27 molecules in the ER

[DOI] [PMC free article] [PubMed] [Google Scholar] 210

These limitations have prompted researchers to explore alternative, non-destructive methods, such as label-free techniques, which offer advantages such as low damage to cells, high sensitivity, and small working volumes (Cunningham & Laing, 2008

Nat Cell Biol 16(11):10691079 Mancias JD, Wang X, Gygi SP, Harper JW, Kimmelman AC (2014) Quantitative proteomics identifies NCOA4 as the cargo receptor mediating ferritinophagy