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Furthermore, this combined treatment strategy augmented the expression of -H2AX and cleaved-caspase 3, indicative of enhanced DNA damage and apoptosis, effects partially reversible by NAC (Figs

Next, 3 g of mouse anti-Strep II-Tag mAb or rabbit anti-DDDDK-tag (Flag) mAb, along with rabbit or mouse control IgG, was coupled to the protein A/G beads in 250 l of IP buffer at 40 C for 4 h on a rotating wheel