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Cells were then lysed by repeated freeze-thaw cycles (three cycles of liquid nitrogen and 37 C water bath), followed by centrifugation at 20,000 g for 20 min at 4 C to separate soluble protein fractions

Altered expression of the clock gene causes a loss of timely coordination among different brain areas, interferes with the regulation of basic cellular metabolic and homeostatic processes, and ultimately results in the disruption of connections among brain structures, ultimately leading to cognitive impairment and the characteristic chrono-disruption seen at the onset of early neurodegenerative diseases in patients

Tuzcu, Mehmet, and Giyasettin Baydas

Cannabinoid oil extract formulations reduce TNF- and enhance brain-derived neurotrophic factor (BDNF) production, improving neurological disability scoring in EAE mice (Zhou et al., 2019)