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Simulation reliability was determined by monitoring energy drift, temperature stability, and pressure at a constant temperature of 300 K and pressure of 1 bar to simulate physiological conditions

[DOI] [PubMed] [Google Scholar] 57.Shankar A.H., Prasad A.S

In short, genomic DNA was extracted by cell lysis (10mM Tris-HCl, 10mM EDTA, 150mM NaCl, 0.1% SDS), proteinase K treatment (New England Biolabs) and DNAse-free RNAse digest (Thermo Fisher), followed by two rounds of phenol extraction and isopropanol precipitation

Despite these challenges, liposomes remain a valuable tool in drug delivery, and ongoing research seeks to maximize their potential while minimizing their burdens [340]