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Participants (n=96) were randomly assigned to once weekly subcutaneous cagrilintide (0.16, 0.30, 0.60, 1.2, 2.4, or 4.5 mg) or injection volume-matched placebo in a 3:1 ratio

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The bacterial cells were grown at 20 C and after 4048 h, the cells were harvested by the centrifugation at 6500 g for 10 min, washed 23 times with phosphate-buffered saline (PBS) buffer (pH 7.2) and the cell pellet was re-suspended in ice cold 20 mM Tris-Cl (pH 7.4)