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Cell pellets were harvested by centrifuging at 4000 rpm, 4 and stored at -20 C

Melatonin was able to effectively promote the secretion of exosomes from adipocytes, increasing the level of exosomal KG derived from adipose tissue

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Diseases associated with RAGE activation include inflammatory diseases, rheumatic or autoimmune diseases, infectious diseases, diabetes, metabolic syndrome and its complications, obesity, insulin resistance, hypertension, atherosclerosis, neurological diseases such as Alzheimers disease, cardiovascular diseases, pulmonary diseases such as chronic obstructive pulmonary disease (COPD), and cancer.[ref] (Yes, that is pretty much every chronic disease that I can think of and all are associated with aging.) Activation of RAGE (cell membrane receptor) causes an increase in reactive oxygen species as well as an increase in inflammatory cytokines