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Immunofluorescent staining in iPSC-derived brain organoids Day 50 and day 100 of cBOs of different conditions embedded in OCT were sectioned at 10 m using the cryotome (Microm HM525, Thermo Fisher Scientific)

In addition to having a different molecular phenotype, type 1 LSECs had a wider luminal space than type 2 LSECs to facilitate a reduction in blood flow velocity into the lobule