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Lancet Neurol 15(5):455532

Samples: 1.0, 0.3, or 0.1 g of 293 cell lysate expressing a GST-tag fusion protein

As a compensation mechanism, tumor cells increase the use of glutamine to fuel carbon skeletons to the KC at the level of 2OGDH ( Increases in the activities of the KC enzymes CS, ACO, isocitrate dehydrogenase (IDH), 2-OGDH, malate dehydrogenase (MDH), besides of PDH have been reported in isolated rat hepatoma mitochondria in comparison to rat liver mitochondria that correlated with an increase in CO 2 production, a product of KC ( The changes in enzyme isoforms expression and enzyme activities in cancer cells suggest possible changes in the steps that exert control on the KC flux compared to non-cancer cells

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