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In this case, the antibody binds to the target and masks a binding site of the naturally occurring ligand, wherein the ligand serves as an effector molecule

Bounous G, Molson JH (2003) The antioxidant system

The recovery of fluorescence representing EDANS-labeled melittin peptide liberated from FQL was assessed by fluorometry at an excitation wavelength of 335 nm and an emission wavelength of 490 nm using a SpectraMAX M5 system (Molecular Devices)