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Therefore, we concluded that activation of p53 effectively prevents APAP accumulation by promoting APAP metabolism and elimination

The operating conditions were 30 C with 50 mM potassium dihydrogen phosphate buffer (pH 2.8) and 10 mM sodium 1-heptanesulfonate as the mobile phase at a flow rate of 1.0 mL/min, and detection was performed with an ultraviolet detector SPD-20A (Shimadzu) at 210 nm

It has been isolated in many different evolutionarily distant organisms such as bacteria (4), plants like Zea mays (5), invertebrates like Dendrocoelum lacteum or Drosophila melanogaster (6, 7)