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Furthermore, SCD1 activity is dependent on the level of unsaturated fatty acids

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S9-mix consisted of 10% (v/v) non-induced hamster or induced rat liver S9-fraction (1 volume S9) and 9 volumes of the respective co-factors (8 mM MgCl 2 , 33 mM KCl, 5 mM glucose-6-phosphate, 4 mM NADP) in sodium orthophosphate buffer (100 mM, pH 7.4)