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Quenched with 4 molar HCl, concentrated, washed (DCM/CH3CN) and purified by preparative HPLC using isocratic 40% MeOH and 60% 0.1%) aqueous formic acid solution gave the desired compounds as a white solid 4 molar HCl was added to make HCl Salt of the final product) after lyophilization

a) Methionine synthase: we have already briefly discussed this possibility above, where homocysteine is converted back into methionine, which is dependent on vitamin B12 and the folate cycle

10.4077/cjp.2009.amh036 12 ChenM.JiangL.LiY.BaiG.ZhaoJ.ZhangM.et al (2017)

You still add the same amount of bacteriostatic water based on the semaglutide content