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1a), we prioritized EMC3, which enabled the purification of ER with markedly reduced contamination from other organelles (Extended Data Fig

This enabled us to remove the metabolic effect from the use of different media

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Cells were plated on 48-well plates, coated with anti-CD3 (1 g ml 1 ) and anti-CD28 (10 g ml ) antibodies, in RPMI medium or DMEM, supplemented with 10% FCS, penicillin, streptomycin, HEPES, glutamine, pyruvate, nonessential amino acids and -mercaptoethanol diluted with PBS (1:1), in the presence of IL-2 (10 ng ml 1 , Peprotech) and IL-12 (10 ng ml 1 , Peprotech) (T H 1 conditions), with or without amino acids