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obtained the 3D structure of glutaminase (GLS) from the protein database and minimized it, screening approximately 60,000 natural compounds from a traditional medicine database, and prepared ligands using the Prepare Ligand Module of Discovery Studio 2020 (Sticht et al., 1994) values of 0.25, 0.33 and 0.31 nm, respectively

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These include fatty acid binding protein (FABP), carnitine palmitoyltransferase 1A (CPT1A), peroxisomal acyl-CoA oxidase, mitochondrial long and medium chain acyl-CoA dehydrogenases (LCAD, MCAD), and the rate-limiting enzyme of ketogenesis, HMGCS2 (Meertens et al., 1998

126 USP20 stabilizes HMG-CoA reductase (HMGCR), the rate-limiting enzyme in cholesterol biosynthesis, and its inhibition reduces lipid levels and improves insulin sensitivity in preclinical models