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Since the simple act of feeding induces ER stress independent of dietary fat content, at least in the liver (Gomez and Rutkowski, 2016), it is possible that the consequences of obesity on ER function depend on whether or not the stimulus is experienced as accelerated feeding and fasting cycles, as in ob/ob mice, or as more calorically potent episodes of feeding with relatively normal frequency

This is why we recommend acclimatizing beforehand

Thus, the role of GSH synthesis in the liver and how this impacts the physiology of adult animals has yet to be fully elucidated

doi: 10.1038/s41392-020-0187-x 250 NishijimaTFShacharSSNyropKAMussHB