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The disease activity of DM was evaluated using the Myositis Disease Activity Assessment Visual Analogue Scales (MYOACT), which was established by the International Myositis Assessment and Clinical Studies (IMACS) group, including constitutional, cutaneous, skeletal, gastrointestinal, pulmonary, cardiovascular, muscle, extramuscular, and a global score

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Both plasma and tissue extracts were centrifuged at 4000 g at 4 C for 10 min, and the supernatant was collected and dried using GeneVac