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Washing is done using a buffer similar to the binding buffer (e.g., TBS) to maintain the binding conditions

Dev Biol 417 , 6376 (2016)

2025 Dec 15:1009:178390

Erythrocytes from knock-out mice that do not express the energy-sensing enzyme AMPK are significantly more susceptible to the eryptotic effect of energy depletion than erythrocytes from wild-type littermates expressing normal levels of AMPK [149]