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Bronchoalveolar lavage (BAL) was performed in 64 asthmatic children (severe asthma, n = 43)

HILIC separation was performed using a Waters BEH Amide column (100 mm 2.1 mm, 1.7 m)

The shoot and root portions of plants were cleansed with sterile water, excess water was eliminated, and they were quickly frozen using liquid nitrogen