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For instance, Liu et al

Most consistent evidence for an impact of opioid drugs on executive function comes from studies implementing the digit symbol substitution task (DSST, also known as the coding task), which requires participants to substitute symbols and digits using a particular key

The cycling profile for real-time PCR (40 cycles) was as follows: 30 s at 95 C for enzyme activation, 5 s at 95 C for initial denaturation, 5 s at 65 C for annealing/extension and a 5 s melt curve step at 65C95 C

Consistent with these findings, DFO and Fer-1 prevented cell death caused by FAC and erastin in IB3-1 cells, as shown by the assessment of cell viability (Fig